Nettle Supplementation Augments the Benefits of Combined Training on blood Glucose control and inflammatory factors in women with type 2 diabetes
Subject Areas : Sport Physiology
Solmaz Babaei
1
*
,
Samaneh Dastah
2
*
1 - Associate Professor of Exercise Physiology, Faculty of Sport Sciences, University of Maragheh, Maragheh, Iran
2 - Assistant Professor of Exercise Physiology, Faculty of Sport Sciences, University of Maragheh, Maragheh, Iran
Keywords: Combined Training, Nettle Supplement, CTRP3, IL-1β , IL-33,
Abstract :
Background: Although consumption of nettle can change the inflammatory response in diabetic patients and may increase the benefits of exercise, no research has yet quantified the simultaneous effect of exercise and consumption of nettle on inflammatory indicators in diabetic patients. This research aims to investigate the effects of 12 weeks of combined training with nettle consumption on inflammatory factors in women with type 2 diabetes
Materials and Methods: The participants (N=60) were randomly distributed into four groups, each consisting of 15 participants: Combined Training group (Com), Nettle Supplement group (NS), Combined Training + Nettle Supplement (Com+NS), Control Group. The intervention comprised a 12-week treatment involving Nettle supplementation, a 12-week combined training (aerobic resistance-periodic) protocol with three sessions per week. Serum levels of blood glucose, insulin, HOMA-IR, CRPT3, IL-1β and IL-33 were measured within 48 h of the before and final training session.
Results: Statistical analyses revealed significant differences across all measures among the groups (p < 0.05). The combined training and supplementation approach led to notable reductions in blood glucose (p=0.001). Furthermore, CTRP3 and IL-33 levels in the three intervention groups were significantly increased compared to the control group (p=0.001). Also, there was a significant decrease in IL-1β levels in the Com, NS, and Com + NS groups relative to the control group (p=0.001).
Conclusion: This investigation underscores the potential of Nettle supplementation and Combined training as a synergistic strategy to ameliorate diabetes-related complications and enhance overall metabolic health in women with type 2 diabetes.
1. Souza KL, Gurgul-Convey E, Elsner M, Lenzen S. Interaction between pro-inflammatory and anti-inflammatory cytokines in insulin-producing cells. J Endocrinol. 2008 Apr;197(1):139-50. doi: 10.1677/JOE-07-0638. PMID: 18372240.
2. Riyahi Malayeri S, Azadniya A, Rasaee M J. Effect of eight-week High intensity interval training nd resveratrol intake on serum adiponectin and resistin in type 2 diabetic rats. Ijdld 2019; 18 (1) :8-1. URL: http://ijdld.tums.ac.ir/article-1-5708-en.html.
3. Riyahi Malayeri S, Kaka Abdullah Shirazi S, Behdari R, mousavi Sadati K. Effect of 8-week Swimming training and garlic intake on serum ICAM and VCAM adhesion molecules in male obese rats. JSSU 2019; 26 (10): 867-878.URL: http://jssu.ssu.ac.ir/article-1-4695-en.html.
4. Martin-Cordero L, Garcia JJ, Giraldo E, De la Fuente M, Manso R, Ortega E. Influence of exercise on the circulating levels and macrophage production of IL-1beta and IFNgamma affected by metabolic syndrome: an obese Zucker rat experimental animal model. Eur J Appl Physiol. 2009 Nov;107(5):535-43. doi: 10.1007/s00421-009-1140-4. Epub 2009 Aug 18. PMID: 19688220.
5. Peiró C, Lorenzo Ó, Carraro R, Sánchez-Ferrer CF. IL-1β Inhibition in Cardiovascular Complications Associated to Diabetes Mellitus. Front Pharmacol. 2017 Jun 13;8:363. doi: 10.3389/fphar.2017.00363. PMID: 28659798; PMCID: PMC5468794.
6. van Asseldonk EJ, Stienstra R, Koenen TB, Joosten LA, Netea MG, Tack CJ. Treatment with Anakinra improves disposition index but not insulin sensitivity in nondiabetic subjects with the metabolic syndrome: a randomized, double-blind, placebo-controlled study. J Clin Endocrinol Metab. 2011 Jul;96(7):2119-26. doi: 10.1210/jc.2010-2992. Epub 2011 Apr 20. PMID: 21508140.
7. Hosseini M, Ghasem Zadeh Khorasani N, Divkan B, Riyahi Malayeri S. Interactive Effect of High Intensity Interval Training with Vitamin E Consumption on the Serum Levels of Hsp70 and SOD in Male Wistar Rats. Iranian J Nutr Sci Food Technol 2019; 13 (4) :21-28URL: http://nsft.sbmu.ac.ir/article-1-2689-en.html.
8. Froozandeh, E., Tofighi, A., Tolouei Azar, J. The Effect of 8 Weeks of Resistance and Aerobic Interval Training on Levels of GLP-1, NRG-1 and IL-33 in Type 2 Diabetic Women. Sport Physiology, 2020; 12(46): 117-138. doi: 10.22089/spj.2019.7434.1913
9. Miller AM, Liew FY. The IL-33/ST2 pathway--A new therapeutic target in cardiovascular disease. Pharmacol Ther. 2011 Aug;131(2):179-86. doi: 10.1016/j.pharmthera.2011.02.005. Epub 2011 Feb 26. PMID: 21356240.
10. Takhti M, Riyahi Malayeri S, Behdari R. Comparison of two methods of concurrent training and ginger intake on visfatin and metabolic syndrome in overweight women. Razi Journal of Medical Sciences. 2020;27(9):98-111.
11. Miller AM, Asquith DL, Hueber AJ, Anderson LA, Holmes WM, McKenzie AN, Xu D, Sattar N, McInnes IB, Liew FY. Interleukin-33 induces protective effects in adipose tissue inflammation during obesity in mice. Circ Res. 2010 Sep 3;107(5):650-8. doi: 10.1161/CIRCRESAHA.110.218867. Epub 2010 Jul 15. PMID: 20634488; PMCID: PMC4254700.
12. Berg A, Scherer P. The Online version of this article, along with updated information and services, is located on the World Wide Web. Journal of the American Heart Association. 2005; 96:939-49.
13. Ouchi N, Kihara S, Funahashi T, Matsuzawa Y, Walsh K. Obesity, adiponectin and vascular inflammatory disease. Curr Opin Lipidol. 2003 Dec;14(6):561-6. doi: 10.1097/00041433-200312000-00003. PMID: 14624132.
14. Peterson JM, Wei Z, Wong GW. C1q/TNF-related protein-3 (CTRP3), a novel adipokine that regulates hepatic glucose output. J Biol Chem. 2010 Dec 17;285(51):39691-701. doi: 10.1074/jbc.M110.180695. Epub 2010 Oct 15. PMID: 20952387; PMCID: PMC3000950.
15. Zhang J, Zhang B, Cheng Y, Xu J. Low serum CTRP3 levels are associated with nonalcoholic fatty liver disease in patients with type 2 diabetes mellitus. Cytokine. 2018 Jun;106:131-135. doi: 10.1016/j.cyto.2017.10.023. Epub 2017 Nov 4. PMID: 29113741.
16. Komosinska-Vassev K, Olczyk P, Kuźnik-Trocha K, Jura-Półtorak A, Derkacz A, Purchałka M, Telega A, Olczyk K. Circulating C1q/TNF-Related Protein 3, Omentin-1 and NGAL in Obese Patients with Type 2 Diabetes During Insulin Therapy. J Clin Med. 2019 Jun 5;8(6):805. doi: 10.3390/jcm8060805. PMID: 31195747; PMCID: PMC6617185.
17. Khademi Z, Imani E, Heidary Khormizi M, Poordad Khodaei A, Sarneyzadeh M, Nikparvar M. A Study on The Variation of Medicinal Plants Used for Controlling Blood Sugar and Causes of Self – Medication by Patients Referred to Bandarabbas Diabetic Center. J Diabetes Nurs 2013; 1 (1) :12-20
18. darabi S, hasanvand A, nourollahi A. Effects of garlic extract, olive leaf extract, nettle extract on serum inflammatory markers hsCRP and IL-6 and TNF-α in rats with diabetes. cmja 2016; 6 (1) :1452-1460.
19. Genc Z, Yarat A, Tunali-Akbay T, Sener G, Cetinel S, Pisiriciler R, Caliskan-Ak E, Altıntas A, Demirci B. The effect of stinging nettle (Urtica dioica) seed oil on experimental colitis in rats. J Med Food. 2011 Dec;14(12):1554-61. doi: 10.1089/jmf.2011.0028. Epub 2011 Aug 23. PMID: 21861725.
20. arighat esfanjani A, Namazi N, Bahrami A, Ehteshami M. Effect of Hydroalcoholic extract of Nettle (Urtica Dioica) on Glycemic Index and Insulin Resistance Index in Type 2 Diabetic Patients. Iranian Journal of Endocrinology and Metabolism 2012; 13 (6) :561-568
21. Jafari, Z., Samani, S.A. & Jafari, M. Insights into the bioactive compounds and physico-chemical characteristics of the extracted oils from Urtica dioica and Urtica pilulifera. SN Appl. Sci. 2, 416 (2020). https://doi.org/10.1007/s42452-020-2219-0
22. Ziaei R, Foshati S, Hadi A, Kermani MAH, Ghavami A, Clark CCT, Tarrahi MJ. The effect of nettle (Urtica dioica) supplementation on the glycemic control of patients with type 2 diabetes mellitus: A systematic review and meta-analysis. Phytother Res. 2020 Feb;34(2):282-294. doi: 10.1002/ptr.6535. Epub 2019 Dec 4. PMID: 31802554.
23. Saeid DabaghMasoud NikbakhtGlycemic Control by Exercise and Urtica Dioica Supplements in Men With Type 2 Diabetes.Jundishapur J Chronic Dis Care.2016;5(1):e31745.https://doi.org/10.17795/jjcdc-31745.
24. Nikroo H, Hosseini SRA, Fathi M, Sardar MA, Khazaei M. The effect of aerobic, resistance, and combined training on PPAR-α, SIRT1 gene expression, and insulin resistance in high-fat diet-induced NAFLD male rats. Physiol Behav. 2020 Dec 1;227:113149. doi: 10.1016/j.physbeh.2020.113149. Epub 2020 Sep 2. PMID: 32888948.
25. Petersen AM, Pedersen BK. The anti-inflammatory effect of exercise. J Appl Physiol (1985). 2005 Apr;98(4):1154-62. doi: 10.1152/japplphysiol.00164.2004. PMID: 15772055.
26. Trøseid M, Lappegård KT, Mollnes TE, Arnesen H, Seljeflot I. The effect of exercise on serum levels of interleukin-18 and components of the metabolic syndrome. Metab Syndr Relat Disord. 2009 Dec;7(6):579-84. doi: 10.1089/met.2009.0003. PMID: 19900154.
27. Nasr Esfahani N, Taghian F. Effect of Eight Weeks of Yoga Training and Zinc Supplementation on the Levels of Interleukin-1 and C-reactive Protein in Women with Type II Diabetes. J Diabetes Nurs 2018; 6 (1) :374-385.
28. Chen HF, Chuang HC, Tan TH. Regulation of Dual-Specificity Phosphatase (DUSP) Ubiquitination and Protein Stability. Int J Mol Sci. 2019 May 30;20(11):2668. doi: 10.3390/ijms20112668. PMID: 31151270; PMCID: PMC6600639.
29. Liu Y, Liu SX, Cai Y, Xie KL, Zhang WL, Zheng F. Effects of combined aerobic and resistance training on the glycolipid metabolism and inflammation levels in type 2 diabetes mellitus. J Phys Ther Sci. 2015 Jul;27(7):2365-71. doi: 10.1589/jpts.27.2365. Epub 2015 Jul 22. PMID: 26311110; PMCID: PMC4540883.
30. Dadvar, N., Ghalavand, A., Zakerkish, M., Hojat, S., Alijani, E., Mahmoodkhanikooshkaki, R. The Effect of Aerobic Training and Urtica Dioica on Lipid Profile and Fasting Blood Glucose in Middle Age Female with Type II Diabetes. Jundishapur Scientific Medical Journal, 2017; 15(6): 507-516.
31. Dadvar N, Ghalavand A, Zakerkish M, Hojat S, Alijani E, Mahmoodkhanikooshkaki R. The effect of aerobic training and Urtica Dioica on lipid profile and fasting blood glucose in middle age female with type II diabetes. Jundishapur Scientific Medical Journal. 2017;15(6):507-16.
32. Hosseinpour Delavar, S., Boyerahmadi, A., Soleymani, A., Ghalavand, A. Effect of eight weeks of aerobic interval training and urtica dioica supplement on some inflammatory indicators and glycemic control in men with type 2 diabetes. Jundishapur Scientific Medical Journal, 2020; 19(2): 123-135. doi: 10.22118/jsmj.2020.200813.1814