The Effects of Coenzyme Q10 Supplementation with Two Consecutive Soccer Games on Stress Oxidative and Muscle Injury Markers in Male Collegiate Soccer Players
Subject Areas : Sport Nutrition
Ehsan Arabzadeh
1
(Exercise Physiology Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran)
Keywords: coenzyme Q10, Soccer Games, Oxidative Stress, Muscle,
Abstract :
Background: This study aimed to investigate the effect of coenzyme Q10 consumption for fourteen days on the response of some indicators of oxidative stress and muscle damage following two consecutive football matches in college players Materials and Methods: for this purpose, 24 football players (with an average age of 20.08±12.1 years, a weight of 63.28±65.1 kg and a maximum oxygen consumption of 53.12± 0.98 ml /per /kg of body weight) The three groups were studied over a fourteen-day period. The control group did not receive these supplements and exercise activities, but the experimental groups included the placebo group (300 mg aspartame) and the coenzyme Q10 group (300 mg ubiquinone supplement) during the course in addition to receiving a placebo or supplement the two 90-minute football matches were less than 48 hours apart. Then, according to the research plan, blood samples were collected from the subjects in two stages 24 hours before and after the period and MDA, LDH, CK and AST indices were measured. Dependent t-test, one-way analysis of variance and Tukey's post hoc test were also used. Results: The results showed a significant difference between serum coenzyme Q 10 supplementation with serum MDA (P = 0.000) and AST (P = 0.006) concentrations from two consecutive football matches, while LDH levels (P = 0.970) and serum CK (P = 0.911) did not change significantly. Conclusion: Overall, the results show that supplementing coenzyme Q10 before and during a two-week match can have anti-oxidant benefits, so it can be recommended to college soccer players.
1. LASS A, SOHAL RS. Effect of coenzyme Q10 and α‐tocopherol content of mitochondria on the production of superoxide anion radicals. The FASEB Journal. 2000;14(1):87-94.doi: 10.1096/fasebj.14.1.87.
PMID: 10627283.
2. Kagan T, Davis C, Lin L, Zakeri Z. Coenzyme Q10 can in some circumstances block apoptosis, and this effect is mediated through mitochondria. Annals of the New York Academy of Sciences. 1999;887(1):31-47.https://doi.org/10.1111/j.1749-6632.1999.tb07920.x
3. Crane FL. Biochemical functions of coenzyme Q10. Journal of the American College of Nutrition. 2001;20(6):591-8.doi:10.1080/07315724.2001.10719063.PMID: 11771674.
4. Overvad K, Diamant B, Holm L, Hølmer G, Mortensen S, Stender S. Coenzyme Q 10 in health and disease. European Journal of Clinical Nutrition. 1999;53(10):764-70.doi: 10.1038/sj.ejcn.1600880.
PMID: 10556981.
5. Andersson H, Bøhn S, Raastad T, Paulsen G, Blomhoff R, Kadi F. Differences in the inflammatory plasma cytokine response following two elite female soccer games separated by a 72‐h recovery. Scandinavian journal of medicine & science in sports. 2010;20(5):740-7.doi:10.1111/j.1600-0838.2009.00989.x. PMID: 19765242.
6. Suzuki K, Yamada M, Kurakake S, Okamura N, Yamaya K, Liu Q, et al. Circulating cytokines and hormones with immunosuppressive but neutrophil-priming potentials rise after endurance exercise in humans. European journal of applied physiology. 2000;81(4):281-7.doi: 10.1007/s004210050044.
PMID: 10664086.
7. Andersson H, Karlsen A, Blomhoff R, Raastad T, Kadi F. Plasma antioxidant responses and oxidative stress following a soccer game in elite female players. Scandinavian journal of medicine & science in sports. 2010;20(4):600-8.
doi: 10.1111/j.1600-0838.2009.00987.x. Epub 2009 Aug 23. PMID: 19706000.
8. Witkowska-Piłaszewicz O, Pingwara R, Winnicka A. The Effect of Physical Training on Peripheral Blood Mononuclear Cell Ex Vivo Proliferation, Differentiation, Activity, and Reactive Oxygen Species Production in Racehorses. Antioxidants (Basel). 2020 Nov 20;9(11):1155. doi: 10.3390/antiox9111155.PMID: 33233549;
PMCID: PMC7699811.
9. Khan SS, Smith MS, Reda D, Suffredini AF, McCoy Jr JP. Multiplex bead array assays for detection of soluble cytokines: comparisons of sensitivity and quantitative values among kits from multiple manufacturers. Cytometry Part B: Clinical Cytometry: The Journal of the International Society for Analytical Cytology. 2004;61(1):35-9.doi: 10.1002/cyto.b.20021. Erratum in: Cytometry B Clin Cytom. 2005 Mar;64(1):53. PMID: 15351980.
10. Martins TB, Pasi BM, Pickering JW, Jaskowski TD, Litwin CM, Hill HR. Determination of cytokine responses using a multiplexed fluorescent microsphere immunoassay. American journal of clinical pathology. 2002;118(3):346-53.doi: 10.1309/N0T6-C56B-GXB2-NVFB.PMID: 12219776.
11. Ostrowski K, Rohde T, Zacho M, Asp S, Pedersen BK. Evidence that interleukin-6 is produced in human skeletal muscle during prolonged running. J Physiol. 1998 May 1;508 ( Pt 3)(Pt 3):949-53. doi: 10.1111/j.1469-7793.1998.949bp.x.PMID: 9518745; PMCID: PMC2230908.
12. Suzuki K, Nakaji S, Yamada M, Liu Q, Kurakake S, Okamura N, et al. Impact of a competitive marathon race on systemic cytokine and neutrophil responses. Medicine & science in sports & exercise.2003;35(2):348-55.doi:10.1249/01.MSS.0000048861.57899.04.PMID: 12569227.
13. Ostrowski K, Rohde T, Asp S, Schjerling P, Pedersen BK. Pro‐and anti‐inflammatory cytokine balance in strenuous exercise in humans. The Journal of physiology. 1999;515(1): 287-91.doi: 10.1111/j.1469-7793.1999.287ad.x.PMID: 9925898; PMCID: PMC2269132.
14. Ascensão A, Rebelo A, Oliveira E, Marques F, Pereira L, Magalhães J. Biochemical impact of a soccer match—analysis of oxidative stress and muscle damage markers throughout recovery. Clinical biochemistry. 2008;41(10-11):841-51.
doi: 10.1016/j.clinbiochem.2008.04.008. Epub 2008 Apr 23. PMID: 18457670.
15. Márcio A B, Juliano S, Cristiana M P, Anna Paola TR P. Oxidative stress biomarkers response to exercise in Brazilian junior soccer players. Food and Nutrition Sciences. 2011; 2011.doi: 10.4236/fns.2011.25057
16. Martinefski MR, Yamasato MF, Di Carlo MB, Daruich JR, Tripodi VP. Coenzyme Q10 deficiency in patients with hereditary hemochromatosis. Clinics and Research in Hepatology and Gastroenterology. 2021;45(6):101624.doi: 10.1016/j.clinre.2021.101624. Epub ahead of print. PMID: 33676282.
17. Kaikkonen J, Tuomainen T-P, Nyyssönen K, Salonen JT. Coenzyme Q10: absorption, antioxidative properties, determinants, and plasma levels. Free radical research. 2002;36(4):389-97.doi: 10.1080/10715760290021234. PMID: 12069102.
18. Akhtar S, Najafzadeh M, Isreb M, Newton L, Gopalan RC, Anderson D. ROS-induced oxidative damage in lymphocytes ex vivo/in vitro from healthy individuals and MGUS patients: protection by myricetin bulk and nanoforms. Archives of toxicology. 2020;94(4):1229-39.
doi: 10.1007/s00204-020-02688-4. Epub 2020 Feb 27. PMID: 32107588; PMCID: PMC7225194.
19. Kadela-Tomanek M, Jastrzębska M, Marciniec K, Chrobak E, Bębenek E, Latocha M, et al. Design, synthesis and biological activity of 1, 4-quinone moiety attached to betulin derivatives as potent DT-diaphorase substrate. Bioorganic Chemistry. 2021; 106:104478.
doi: 10.1016/j.bioorg.2020.104478. Epub 2020 Nov 19. PMID: 33272711.
20. Wang R, Cai K, Wang H, Yin C, Cheng J. A caged metabolic precursor for DT-diaphorase-responsive cell labeling. Chemical Communications. 2018;54(38):4878-81.
doi: 10.1039/c8cc01715h. PMID: 29700507; PMCID: PMC6508093.
21. Radak Z, Ogonovszky H, Dubecz J, Pavlik G, Sasvari M, Pucsok J, et al. Super‐marathon race increases serum and urinary nitrotyrosine and carbonyl levels. European journal of clinical investigation. 2003;33(8):726-30.
Doi: 10.1046/j.1365-2362.2003. 01202.x